欢迎访问云南西力生物技术股份有限公司!证券代码:871574
首页 成 果 源于西力产品的客户论文
Ellagic Acid Blocks RANKL–RANK Interaction and Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting RANK Signaling Pathways. Chem. Biol. Interact., 2020, 331: 109235.
发布时间:2020-10-22 阅读数:324 来源:Chem. Biol. Interact.
分享到:

Abstract:

Ellagic acid (EA) is a naturally occurring polyphenolic compound that has been shown to exhibit diverse beneficial pharmacological activities including anti-osteoclastogenesis effect. However, the molecular mechanism by which EA inhibits osteoclastogenesis remains to be elucidated. The protein-protein interaction between receptor activator of nuclear factor (NF)-κB ligand (RANKL) and its receptor RANK contributes to osteoclast differentiation and activation in bone remodeling, and is regarded as an important therapeutic target for the treatment of osteoporosis. The current study is focused on investigating whether EA can directly bind to RANKL and/or RANK and block the interaction between RANKL and RANK, thereby inhibiting downstream signaling pathways. Interestingly, we found that EA had strong affinities to RANK and RANKL, with the estimated equilibrium dissociation constants (KD) of 2.485 × 10-11 and 1.688 × 10-9 M, respectively, and could disrupt the interaction between RANKL and RANK, thereby inhibiting RANKL-induced canonical RANK signaling pathways (p65, JNK, ERK, and p38) and expression of downstream master transcriptional factors (NFATc1 and c-Fos) and osteoclast-specific genes and proteins (TRAP, c-Src, and cathepsin K), which could ultimately suppress RANKL-induced osteoclast differentiation and F-actin ring formation. Taken together, our results revealed that EA could block RANKL-RANK interaction and suppress RANKL-induced osteoclastogenesis by inhibiting RANK signaling pathways in RAW 264.7 murine macrophages.



ewm.jpg 微信公众号
11.jpg 移动官网
特别声明:禁止在未经同意情况下转载本网站信息,BioBioPha品牌产品均由西力生物独家生产与销售,仅用于科学研究或企业研发!
版权所有 2019-2020 云南西力生物技术股份有限公司 BioBioPha 滇ICP备09000810号-1滇公安备案号 53019002000069号
技术支持:奥远科技

联系客服